Identification and characterization of conserved miRNAs with its targets mRNA in Trichinella Spiralis
نویسندگان
چکیده
microRNAs (Small regulatory non-coding RNAs) have an important role in gene regulation and evolutionarily conserved molecules. Trichinella spiralis infect majority of species. Therefore, it is of interest to identify conserved miRNAs and their targets using sequences from EST, GSS and full length nucleotides obtained from NCBI against previously reported worm miRNAs. We identify 11 novel miRNAs in T. spiralis by using bioinformatics-homology based search. In addition, we predicted target mRNA genes form complementary base pair in seed region of miRNAs. Further, gene annotation using Uniprot shows that these target genes of miRNAs are involved in various metabolism, enzymatic activity and constituents of membrane components.
منابع مشابه
Transcriptome of Small Regulatory RNAs in the Development of the Zoonotic Parasite Trichinella spiralis
BACKGROUND Trichinella spiralis is a parasite with unique features. It is a multicellular organism but with an intracellular parasitization and development stage. T. spiralis is the helminthic pathogen that causes zoonotic trichinellosis and afflicts more than 10 million people worldwide, whereas the parasite's biology, especially the developmental regulation is largely unknown. In other organi...
متن کاملCharacterisation of the Trichinella spiralis Deubiquitinating Enzyme, TsUCH37, an Evolutionarily Conserved Proteasome Interaction Partner
BACKGROUND Trichinella spiralis is a zoonotic parasitic nematode that causes trichinellosis, a disease that has been identified on all continents except Antarctica. During chronic infection, T. spiralis larvae infect skeletal myofibres, severely disrupting their differentiation state. METHODOLOGY AND RESULTS An activity-based probe, HA-Ub-VME, was used to identify deubiquitinating enzyme (DUB...
متن کاملBioinformatics identification of miRNA-mRNA regulatory network contributing to lung cancer invasion
Background: Over the past 15 years, significant insights have been gained into the roles of miRNAs in cancer. In various cancers, miRNAs can act as oncogenes, tumor suppressors, or control the metastasis process by modulating the expression of numerous target genes. This study is aimed at determining molecular network of miRNA-mRNA regulating lung cancer invasion, by bioinformatics approaches. ...
متن کاملP-71: Construction of Required DNA Plasmids for Validation of Predicted MicroR-NA Targets
Background: The micro-ribonucleic acids (miRNAs) are noncoding RNA molecules that are conserved developmentally and include usually 18-25 nucleotides. MiRNA regulates gene expression through mRNA degradation or inhibiting of its translation. These biomolecules contribute in cellular physiologic and pathologic processes and most of them may act as oncogenes or tumor inhibitors. Identification of...
متن کاملBioinformatics Identification of miRNA-mRNA Regulatory Network Contributing Primary Lung Cancer
Introduction: In clinical practice, distinguishing invasive lung tumors from primary tumors remains a challenge. With recent advances in understanding biological alterations of tumorigenesis and molecular analytic technologies, using these molecular alterations can be sensitive and tumor-specific as biomarker for the stratification of patients. In this study, the molecular network of miRNA-mRNA...
متن کامل